32.8 Application of Metagenomics

503

Table 32.3

Metagenomics insights into microbial ecology during anaerobic digestion of diverse

feedstock.

Feedstock

Metagenomics tool

Microbial community

AD condition

References

Food waste

High-throughput

16S rRNA gene

sequencing

Shotgun

metagenomics

sequencing

Coprothermobacter

proteolyticus, Genus

Thermacetogenium,

Dictyoglomus

thermophilum,

Thermodesulfovibrio

yellowstonii,

Syntrophomonas wolfei,

Anaerobaculum spp.

60 ^∘C

[13]

Cow dung,

mixed fruit

and vegetable

waste

PCR-DGGE

High-throughput

sequence

analyses

Methanosarcina, Phylum

Bacteroidetes, Firmicutes,

Actinobacteria,

Proteobacteria, Class

Bacteroidia, Clostridia,

Genus Syntrophomonas,

Methanobrevibacter,

Methanobacterium

Co-digestion

Semi-continuous

stir tank

reactors, 30 ^∘C

[4]

Municipal

sludge

Industrial

wastewater

DNA Extraction

and Illumina

Sequencing

Methanothermobacter,

Methanospirillum,

Methanobrevibacter,

Thermococcus

—

[14]

Food waste

Dairy cow

manure

16S rRNA gene

sequencing

Chloroflexi,

Methanosaeta,

Methanothermobacter,

Thermotogae, Firmicutes,

and Synergistetes

Methanobacterium and

Methanosaeta

Methanothermobacter,

Thermotogae

Mesophilic

digestion

Thermophilic

digestion

Mesophilic

co-digestion

Thermophilic

co-digestion

[15]

Organic

fraction MSW,

maize silage

and cow dung

waste, mixed

waste, brewery

waste water

Metaproteomics

Bacillales,

Enterobacteriales,

Bacteriodales,

Clostridiales, Rhizobiales,

Thermoanaerobacteri-

ales, Methanobacteriales,

Methanosarcinales, and

Methanococcales

UASB fermenter,

Mesophilic and

Thermophilic

temperature

[16]

Cattle manure

High-throughput

shotgun

sequencing

Novel binning

strategy

Phylum Firmicutes,

Proteobacteria and

Bacteroidetes

Eubacteriaceae,

Syntrophomonadaceae,

Class Clostridia, Genus

Methanoculleus

CSTR at 54 ^∘C

[17]